Occurrence of Bovine Mastitis Caused by CTX-M-2 <I>β</I>-Lactamase Producing <I>Klebsiella pneumoniae</I>

DOI JASI Web Site 18 References
  • OHNISHI Mamoru
    Veterinary Clinical Laboratory, Nemuro District Agricultural Mutual Aid Association
  • SAWADA Takuo
    Department of Veterinary Microbiology, School of Veterinary Medicine, Nippon Veterinary and Life Science University
  • HARADA Kazuki
    Department of Veterinary Internal Medicine, Tottori University
  • ESAKI Hidetake
    Department of Biotechnology, Research Institute for Animal Science in Biochemistry and Toxicology
  • SHIMURA Keiko
    Department of Biotechnology, Research Institute for Animal Science in Biochemistry and Toxicology
  • MARUMO Kenji
    Department of Microbiology and Immunology, Showa University School of Medicine
  • TAKAHASHI Toshio
    Department of Veterinary Microbiology, School of Veterinary Medicine, Nippon Veterinary and Life Science University

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Other Title
  • CTX-M-2型 <I>β</I>-ラクタマ－ゼ産生<I>Klebsiella pneumoniae</I>による牛乳房炎の発生
  • Occurrence of Bovine Mastitis Caused by CTX-M-2 β-Lactamase Producing Klebsiella pneumoniae
  • Occurrence of Bovine Mastitis Caused by CTX-M-2 ^|^beta;-Lactamase Producing Klebsiella pneumoniae

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Abstract

The aims of this study were to determine the presence of Enterobacteriaceae strains producing CTX-M-type extended-spectrum β-lactamases (ESBLs) (CTX-M) involved in bovine mastitis among Japanese dairy cattle and clinical courses of cows affected by the mastitis. Between August 2006 and January 2007 we chose 51 cefazolin-resistant isolates from oxidase-negative, Gram-negative bacilli isolates obtained from 30,237 quarter milk samples from 20,194 cows with mastitis in 1,000 dairy farms in Nemuro Subprefecture of Hokkaido Prefecture, Japan. They were screened for ESBLs using the standard Clinical and Laboratory Standards Institute (CLSI) ESBLs confirmatory combination disc tests. We performed genotyping of CTX-M-, TEM-, and SHV-type β-lactamases by PCR analysis and nucleotide sequencing, and determined the minimum inhibitory concentrations (MICs) of 21 antimicrobials. We identified three Klebsiella pneumoniae isolates producing CTX-M-2 from three quarters of two cows with mastitis in two dairy farms. One cow had mild acute clinical mastitis (slight swelling, warmth and hardness of the udder, slightly watery foremilk with flakes) without systemic symptoms and resolved within 4 weeks of diagnosis. Another cow had severe acute clinical mastitis with systemic symptoms and resolved within 10 weeks of diagnosis. The three isolates exhibited resistance to ampicillin, cefazolin, cefuroxime, cefotaxime, ceftriaxone, cefpodioxime, ceftiofur, cefquinome, kanamycin, and oxytetracycline. Conversely, they were susceptible to ceftazidime, cefmetazole, moxalactam, imipenem, aztreonam, gentamicin, trimethoprim-sulfamethoxazole, and enrofloxacin. This is the first report on CTX-M-producing K. pneumoniae involved in bovine mastitis in Japan.

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