Serious Limitation of Preimplantation Genetic Diagnosis.
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- Nishimura Takayo
- 1st Department of Obstetrics and Gynecology, School of Medicine, Toho University
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- Sasabe Yutaka
- 1st Department of Obstetrics and Gynecology, School of Medicine, Toho University
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- Shibui Yukihiro
- 1st Department of Obstetrics and Gynecology, School of Medicine, Toho University
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- Ito Kanako
- 1st Department of Obstetrics and Gynecology, School of Medicine, Toho University
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- Katagiri Yukiko
- 1st Department of Obstetrics and Gynecology, School of Medicine, Toho University
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- Masaki Kazuo
- 1st Department of Obstetrics and Gynecology, School of Medicine, Toho University
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- Abe Yuji
- 1st Department of Obstetrics and Gynecology, School of Medicine, Toho University
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- Kubo Harumi
- 1st Department of Obstetrics and Gynecology, School of Medicine, Toho University
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Abstract
Preimplantation genetic diagnosis (PGD) is the technology used to avoid inheritance of genetic disease by selecting unaffected embryos. Furthermore PGD has made genetic screening of embryos possible. The limited number of cells available for genetic tests has been a serious problem with PGD. The first solution was the application of molecular technologies such as PCR, FISH and cell recycling. The next solution was the visualization of metaphase plates and application of advanced FISH technologies. The ideal solution was in vitro culture of blastomeres to increase the number of cells because it would be easy to reexamine them to assure the accuracy of genetic test results and obtain additional genetic information. The culture system for ES cells was applied to culturing mouse isolated blastomeres because it met two requirements: rapid proliferation and maintenance of the normal karyotype during culture. Our trial with mouse embryos successfully demonstrated these two requirements.
Journal
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- Journal of Mammalian Ova Research
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Journal of Mammalian Ova Research 19 (1), 1-5, 2002
JAPANESE SOCIETY OF OVA RESEARCH
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Details 詳細情報について
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- CRID
- 1390282679316175616
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- NII Article ID
- 130004436647
- 10012144312
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- NII Book ID
- AN10548943
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- ISSN
- 13475878
- 13417738
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- NDL BIB ID
- 6147319
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed