透明帯開孔術後卵子 (ハムスター, ヒト) の走査型電子顕微鏡による観察

井上 正人, 松井 素子, 小林 善宗, 本田 育子, 淡路 英雄, 津田 朋男, 藤井 明和

doi:10.14893/jmor1984.6.143

透明帯開孔術後卵子 (ハムスター, ヒト) の走査型電子顕微鏡による観察

DOI

井上正人

Department of Obstetrics and Gynecology, School of Medicine, Tokai University
松井素子

Department of Obstetrics and Gynecology, School of Medicine, Tokai University
小林善宗

Department of Obstetrics and Gynecology, School of Medicine, Tokai University
本田育子

Department of Obstetrics and Gynecology, School of Medicine, Tokai University
淡路英雄

Department of Obstetrics and Gynecology, School of Medicine, Tokai University
津田朋男

Department of Obstetrics and Gynecology, School of Medicine, Tokai University
藤井明和

Department of Obstetrics and Gynecology, School of Medicine, Tokai University

書誌事項

タイトル別名

Scanning Electron Microscopic Observation of Zona Drilled Hamster and Human Oocytes

この論文をさがす

CiNii Books

説明

Zona drilled hamster and human oocytes were examined by scanning electron microscope. In the hamster mechanical drilling by micropipette created a beautiful hole mimicking sperm penetration hole left on the zona. Chemical drilling with acidic solusion (PH 2.2) made a round, straight hole with the size corresponding to the micropipette used. In the human oocytes, mechanical drilling formed a round, somewhat irregular, micropipette sized hole. Neither the zona surface, nor the microvilli were damadged. On the contrary, drilling with ascidic solusion resulted in the formation of a large, V-shaped hole, reflecting the different solubility properties of human zona pellucida. The microvilli on the plasma membrane were apparently affected. These results indicate that a simple drilling by micropipette is enough to create a hole sufficient for sperm passage, and that acidic solusion is not recommended for human zona drilling because of potential damadge to the vitellus.