-
- HASSANEIN Raafat
- Nippon Veterinary and Life Science University, Laboratory of Veterinary Microbiology
-
- 鈴木 吉一
- 山形県内陸食肉衛生検査所
-
- 澤田 拓士
- 日本獣医生命科学大学獣医微生物学教室
書誌事項
- タイトル別名
-
- Epidemiological Analysis of Erysipelothrix Isolates from Various Animals by Acriflavine Resistance and RAPD Typing
この論文をさがす
抄録
The epidemiological analysis of Erysipelothrix isolates recovered from pigs, cattle and chickens was studied by the analysis of acriflavine resistance and the PCR-based DNA fingerprinting method using random amplified polymorphic DNA (RAPD). Thirty-two Erysipelothrix field isolates, 7 Erysipelothrix reference strains and 13 random primers were tested. Among the tested primers, the primers NK6 (CCCGCGCCCC) and D9355 (CCGGATCCGTGATGCGGTGCG) produced noticeable results. The primer NK6 revealed 5 RAPD patterns (a∼e) while primer D9355 revealed 8 RAPD patterns (A∼H) that were not serovar specific. Namely, different patterns were produced among strains of the same serovar showing that the RAPD method is able to identify the genetic variations of Erysipelothrix species but the RAPD data demonstrated that the some serovar 1a E. rhusiopathiae strains including strain Koganei 65-0.15 for the production of live vaccine were closely related each other genetically, irrespective of their acriflavine resistance. Based on these results, we concluded that the RAPD method with primer D9355 is a rapid and reliable method to differentiate Erysipelothrix isolates from various animals ; and might be a useful tool for the epidemiological analysis of the Erysipelothrix species.
収録刊行物
-
- 獣医疫学雑誌
-
獣医疫学雑誌 11 (1), 23-31, 2007
獣医疫学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679316868736
-
- NII論文ID
- 10022001652
- 40015601360
-
- NII書誌ID
- AA11157349
-
- ISSN
- 18812562
- 09128913
- 13432583
-
- NDL書誌ID
- 8915153
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可