ブタ原始卵胞の生存性に及ぼすKIT ligandの影響
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- Magamage Manjula P. S.
- Laboratory of Reproductive Biology and Biotechnology, Graduate School of Agricultural Science, Kobe University
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- Moniruzzaman Mohammad
- Laboratory of Reproductive Biology and Biotechnology, Graduate School of Agricultural Science, Kobe University
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- Miyano Takashi
- Laboratory of Reproductive Biology and Biotechnology, Graduate School of Agricultural Science, Kobe University
書誌事項
- タイトル別名
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- Effect of KIT Ligand on the Viability of Porcine Primordial Follicles In Vitro
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抄録
In mammals, the mechanisms regulating the initiation of follicle development remain poorly understood. We previously reported that testosterone induced the activation of porcine primordial follicles after 7 days of culture. Herein, the effect of KIT ligand (KL) on the viability, activation and development of porcine primordial follicles was examined. Ovarian strips containing primordial follicles were cultured for 7 days in a medium supplemented with KL (10, 100, 250 or 500 ng/ml). The percentage of degenerated follicles was significantly decreased (39 ± 2%) in the 100 ng/ml KL-treated group compared to the control (56 ± 1%), whereas almost all of the primordial follicles degenerated at higher concentrations of KL. No primordial follicles developed in culture at any concentration of KL. When ovarian strips were cultured in medium supplemented with testosterone (10–6 M) + KL (100 ng/ml), primordial follicles developed to early secondary follicles (5 ± 3%), however the total percentages of developing follicles in the testosterone group (21 ± 5%) and the testosterone + KL group (28 ± 5%) were not significantly different. These results suggest that KL does not promote primordial follicle activation, although it does promote follicle viability in culture.<br>
収録刊行物
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- 日本哺乳動物卵子学会誌
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日本哺乳動物卵子学会誌 28 (1), 61-67, 2011
日本卵子学会
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詳細情報 詳細情報について
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- CRID
- 1390282679317002368
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- NII論文ID
- 10028124082
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- NII書誌ID
- AN10548943
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- ISSN
- 13475878
- 13417738
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- NDL書誌ID
- 11105098
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可