Pathogenesis of IgG4-related disease-Molecular biological approaches
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- Tsuboi Hiroto
- Department of Internal Medicine, Faculty of Medicine, University of Tsukuba
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- Iizuka Mana
- Department of Internal Medicine, Faculty of Medicine, University of Tsukuba
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- Takahashi Hiroyuki
- Department of Internal Medicine, Faculty of Medicine, University of Tsukuba
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- Asashima Hiromitsu
- Department of Internal Medicine, Faculty of Medicine, University of Tsukuba
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- Hirota Tomoya
- Department of Internal Medicine, Faculty of Medicine, University of Tsukuba
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- Kondo Yuya
- Department of Internal Medicine, Faculty of Medicine, University of Tsukuba
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- Nakai Yuji
- Functional Food Science and Nutrigenomics, Graduate School of Agricultural and Life Sciences, The University of Tokyo Institute for Food Sciences, Hirosaki University
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- Abe Keiko
- Functional Food Science and Nutrigenomics, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Tanaka Akihiko
- Faculty of Dental Science, Kyushu University
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- Moriyama Masafumi
- Faculty of Dental Science, Kyushu University
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- Nakamura Seiji
- Faculty of Dental Science, Kyushu University
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- Yoshihara Toshio
- Department of Otorhinolaryngology, Tokyo Women's Medical University
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- Matsumoto Isao
- Department of Internal Medicine, Faculty of Medicine, University of Tsukuba
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- Sumida Takayuki
- Department of Internal Medicine, Faculty of Medicine, University of Tsukuba
Bibliographic Information
- Other Title
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- IgG4関連疾患の病因―分子生物学的アプローチ―
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Abstract
<p><Objective> To compare gene expression in IgG4-related disease (IgG4-RD) with Sjögren’s syndrome (SS) comprehensively, and to identify disease-related molecules.<br><Methods> Gene expression was analyzed by DNA microarray in labial salivary gland (LSG) of IgG4-RD (n=5), SS (n=5), and healthy controls (HC) (n=3). Gene expression patterns were compared by principal component analysis (PCA), and differentially expressed genes (DEGs) between IgG4-RD and SS were identified in pairwise comparisons. Validation of the result was performed by quantitative PCR and immunofluorescence (IF) staining for highly expressed DEGs in IgG4-RD than in SS.<br><Results> Gene expression patterns in IgG4-RD, SS, and HC were quite different with each other in PCA. In IgG4-RD, 1321 up-regulated and 1320 down-regulated genes compared with SS were identified as DEGs. Quantitative PCR validated significantly higher expression of chemokine (C-C motif) ligand 18 (CCL18) which has chemotactic activity on various lymphocytes and induces collagen production from fibroblasts, and lactotransferrin (LTF) which is an abundant iron-binding protein in milk and has the wide range of functions such as the maturation of dendritic cells (DCs), in LSG of IgG4-RD than in that of SS. IF staining clarified that CCL18 was specifically highly expressed in LSG of IgG4-RD, compared with those of SS and HC. Macrophages, DCs, B cells, and plasmacytes expressed CCL18 in LSG of IgG4-RD.<br><Conclusion> These results clearly showed that the gene expression pattern in LSG of IgG4-RD is different from that of SS. CCL18 and LTF were identified as disease-related molecules of IgG4-RD.</p>
Journal
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- Clinical Rheumatology and Related Research
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Clinical Rheumatology and Related Research 29 (2), 128-139, 2017
The Japanese Society for Clinical Rheumatology and Related Research
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Keywords
Details 詳細情報について
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- CRID
- 1390282679318118400
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- NII Article ID
- 130006068221
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- ISSN
- 21890595
- 09148760
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed