Mechanism of the protective effect of high molecular weight hyaluronic acid against cartilage degeneration

DOI
  • Harada Mari
    Department of Pathology, Iwate Medical University, School of Medicine
  • Uzuki Miwa
    Department of Pathology, Iwate Medical University, School of Medicine Faculty of Medical Science and Welfare, Tohoku Bunka Gakuen University
  • Naoki Ishiguro
    Department of Orthopedics, Nagoya University, Graduate School of Medicine
  • Iwadate Katsuji
    New Drug Research Center. Inc
  • Sawai Takashi
    Department of Pathology, Iwate Medical University, School of Medicine Sendai Open Hospital, Sendai city Medical care Center Department of Pathology, Tohoku University, Graduate School of Medicine

Bibliographic Information

Other Title
  • 高分子ヒアルロン酸（HA）による軟骨保護作用のメカニズムの解明

Search this article

Description

Purpose: The therapeutic effect of hyaluronic acid (HA) on osteoarthritis (OA) was assessed in Kb1 rabbits by histopathological examination.<br> Materials: An OA model was prepared in rabbits according to Ishiguro’s method. Then an HA preparation (SUVENYLⓇ HA [ MW 2.7 million Da] or SynviscⓇ HA [mixture of cross-linked HA prepared from rooster’ combs]) or saline was repeatedly injected into the left knee joint of the each rabbit. Knee joint tissues were fixed in 10% formalin and paraffin embedded specimens were routinely processed for histological examination.<br> Methods: The therapeutic effect of HA on joint cartilage was assessed from the following three sets of parameters: (1) maintenance of proteoglycan, HA, or type II collagen; (2) degeneration of the extra cellular matrix and cartilage, assessed by observing aggrecan degradation products or apoptotic cells; and (3) remodeling evaluated by staining with Ki67 or detection of hyaluronan synthase (HAS) 2 or 3 mRNA.<br> Results: The histological grade of OA for the tibial compartment was 4.81 ± 0.25 in the saline group, and 4.69 ± 0.25 in the SVENYLⓇ HA-treated group, and 4.77 ± 0.26 in the SynviscⓇ HA-treated group, while the grade for femoral compartment were 4.69 ± 0.25, 4.35 ± 0.43 and 4.38 ± 0.30, respectively. The residual levels of proteoglycan, type II collagen, and HA were increased in the femurs of the two HA- treated groups compared with the saline group. In addition, the positive ratio of apoptosis and aggrecan degradation products were significantly decreased in the HA-treaed groups compared with the saline group. Moreover, the number of Ki67 positive cells and the expression of HAS2 and HAS3 mRNAs were significantly greater in HA-treated groups than in the saline group. In the tibia, the trends of these parameters were similar to those for femur, but differences among the three groups were smaller.<br> Conclusions: Efficacy of HA was demonstrated by a reduction of parameters indicating cartilage damage, and an increase of parameters indicating a remodeling effect on cartilage.

Journal

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top