Visualization of Sialic Acid Produced on Bacterial Cell Surfaces by Lectin Staining
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- Kajiwara Hitomi
- Glycotechnology Business Unit, Japan Tobacco Inc.
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- Toda Munetoyo
- Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University
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- Mine Toshiki
- Glycotechnology Business Unit, Japan Tobacco Inc.
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- Nakada Hiroshi
- Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University
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- Wariishi Hiroyuki
- Department of Forest and Forest Products Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyusyu University
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- Yamamoto Takeshi
- Glycotechnology Business Unit, Japan Tobacco Inc.
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説明
Oligosaccharides containing N-acetylneuraminic acid on the cell surface of some pathogenic bacteria are important for host-microbe interactions. N-acetylneuraminic acid (Neu5Ac) plays a major role in the pathogenicity of bacterial pathogens. For example, cell surface sialyloligosaccharide moieties of the human pathogen Haemophilus influenzae are involved in virulence and adhesion to host cells. In this study, we have established a method of visualizing Neu5Ac linked to a glycoconjugate on the bacterial cell surface based on lectin staining. Photobacterium damselae strain JT0160, known to produce a-2,6-sialyltransferase, was revealed to possess Neu5Ac by HPLC. Using the strain, a strong Sambucus sieboldiana lectin-binding signal was detected. The bacteria producing α-2,6-sialyltransferases could be divided into two groups: those with a lot of α-2,6-linked Neu5Ac on the cell surface and those with a little. In the present study, we developed a useful method for evaluating the relationship between Neu5Ac expression on the cell surface and the degree of virulence of marine bacteria.<br>
収録刊行物
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- Microbes and environments
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Microbes and environments 25 (3), 152-155, 2010
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282679321478144
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- NII論文ID
- 10026643122
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- NII書誌ID
- AA11173196
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- ISSN
- 13474405
- 13426311
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- NDL書誌ID
- 10810438
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- PubMed
- 21576867
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- 本文言語コード
- en
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- データソース種別
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- NDLサーチ
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