Molecular Characterization of the Bacterial Community in a Potato Phytosphere

DOI Web Site Web Site PubMed 11 Citations 122 References
  • Someya Nobutaka
    Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization
  • Ohdaira Kobayashi Yuki
    Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization
  • Tsuda Shogo
    Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization
  • Ikeda Seishi
    Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization

Search this article

Abstract

The bacterial community of a potato phytosphere at the flowering stage was examined using both culture-dependent and -independent methods. Tissues (leaves, stems, roots and tubers) were sampled from field-grown potato plants (cultivar Matilda), and the clone libraries of 16S rRNA genes and the isolate collections using R2A medium were constructed. By analyzing the combined data set of 16S rRNA gene sequences from both clone libraries and isolate collections, 82 genera from 8 phyla were found and 237 OTUs (≥97% identity) at species level were identified across the potato phytosphere. The statistical analyses of clone libraries suggested that stems harbor the lowest diversity among the tissues examined. The phylogenetic analyses revealed that the most dominant phylum was shown to be Proteobacteria for all tissues (62.0%–89.7% and 57.7%–72.9%, respectively), followed by Actinobacteria (5.0%–10.7% and 14.6%–39.4%, respectively). The results of principal coordinates analyses of both clone libraries and isolate collections indicated that distinct differences were observed between above- and below-ground tissues for bacterial community structures. The results also revealed that leaves harbored highly similar community structures to stems, while the tuber community was shown to be distinctly different from the stem and root communities.<br>

Journal

  • Microbes and Environments

    Microbes and Environments 28 (3), 295-305, 2013

    Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Citations (11)*help

See more

References(122)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top