Occurrence of Escherichia coli O157:H7 in river water determined by flow cytometry.

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  • TANAKA YASUNOBU
    Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University
  • YOSHIMITSU MASATO
    Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University
  • YAMAGUCHI NOBUYASU
    Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University
  • TANI KATSUJI
    Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University
  • NASU MASAO
    Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University

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タイトル別名
  • Occurrence of Escherichia coli O157 H7
  • H7 in river water determined by flow cytometry

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Flow cytometry with a fluorescent antibody technique was applied to detect Escherichia coli O157: H7. Samples from the Neya River in Osaka Prefecture, Japan, were analyzed by flow cytometry to determine the number of E. coli O157: H7 in river water. The numbers of E. coli O157: H7 cells in the river water samples ranged from 5×103 cells/ml to 6×104 cells/ml. These antibody-positive cells grew during cultivation in modified EC medium. These results indicate that viable E. coli O157: H7 occurs in river water.

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