Presence of a Haloarchaeal Halorhodopsin-Like Cl⁻ Pump in Marine Bacteria
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- Nakajima Yu
- Atmosphere and Ocean research Institute (AORI), The University of Tokyo Department of Natural Environmental Studies, Graduate School of Frontier Sciences, the University of Tokyo
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- Tsukamoto Takashi
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Kumagai Yohei
- Atmosphere and Ocean research Institute (AORI), The University of Tokyo Department of Natural Environmental Studies, Graduate School of Frontier Sciences, the University of Tokyo
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- Ogura Yoshitoshi
- Department of Bacteriology, Faculty of Medical Sciences, Kyushu University
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- Hayashi Tetsuya
- Department of Bacteriology, Faculty of Medical Sciences, Kyushu University
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- Song Jaeho
- Department of Biological Sciences, Inha University
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- Kikukawa Takashi
- Faculty of Advanced Life Science, Hokkaido University Global Station for Soft Matter, Global Institution for Collaborative Research and Education, Hokkaido University
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- Demura Makoto
- Faculty of Advanced Life Science, Hokkaido University Global Station for Soft Matter, Global Institution for Collaborative Research and Education, Hokkaido University
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- Kogure Kazuhiro
- Atmosphere and Ocean research Institute (AORI), The University of Tokyo Department of Natural Environmental Studies, Graduate School of Frontier Sciences, the University of Tokyo
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- Sudo Yuki
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Yoshizawa Susumu
- Atmosphere and Ocean research Institute (AORI), The University of Tokyo Department of Natural Environmental Studies, Graduate School of Frontier Sciences, the University of Tokyo
書誌事項
- タイトル別名
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- Presence of a Haloarchaeal Halorhodopsin-Like Cl<sup>−</sup> Pump in Marine Bacteria
- Presence of a Haloarchaeal Halorhodopsin-Like Cl
- Presence of a haloarchaeal halorhodopsin-like Cl- pump in marine bacteria
この論文をさがす
説明
<p>Light-driven ion-pumping rhodopsins are widely distributed among bacteria, archaea, and eukaryotes in the euphotic zone of the aquatic environment. H+-pumping rhodopsin (proteorhodopsin: PR), Na+-pumping rhodopsin (NaR), and Cl−-pumping rhodopsin (ClR) have been found in marine bacteria, which suggests that these genes evolved independently in the ocean. Putative microbial rhodopsin genes were identified in the genome sequences of marine Cytophagia. In the present study, one of these genes was heterologously expressed in Escherichia coli cells and the rhodopsin protein named Rubricoccus marinus halorhodopsin (RmHR) was identified as a light-driven inward Cl− pump. Spectroscopic assays showed that the estimated dissociation constant (Kd,int.) of this rhodopsin was similar to that of haloarchaeal halorhodopsin (HR), while the Cl−-transporting photoreaction mechanism of this rhodopsin was similar to that of HR, but different to that of the already-known marine bacterial ClR. This amino acid sequence similarity also suggested that this rhodopsin is similar to haloarchaeal HR and cyanobacterial HRs (e.g., SyHR and MrHR). Additionally, a phylogenetic analysis revealed that retinal biosynthesis pathway genes (blh and crtY) belong to a phylogenetic lineage of haloarchaea, indicating that these marine Cytophagia acquired rhodopsin-related genes from haloarchaea by lateral gene transfer. Based on these results, we concluded that inward Cl−-pumping rhodopsin is present in genera of the class Cytophagia and may have the same evolutionary origins as haloarchaeal HR.</p>
収録刊行物
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- Microbes and environments
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Microbes and environments 33 (1), 89-97, 2018
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282679322171520
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- NII論文ID
- 130006581777
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- NII書誌ID
- AA11551577
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- ISSN
- 13474405
- 13426311
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- NDL書誌ID
- 028909688
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- PubMed
- 29553064
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- 本文言語コード
- en
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- 資料種別
- journal article
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- NDLサーチ
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- PubMed
- CiNii Articles
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