Bioconversion of Styrene to Poly(hydroxyalkanoate) (PHA) by the New Bacterial Strain <i>Pseudomonas putida</i> NBUS12

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  • Tan Giin-Yu Amy
    Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University
  • Chen Chia-Lung
    Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University
  • Ge Liya
    Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University
  • Li Ling
    Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University
  • Tan Swee Ngin
    Natural Sciences and Science Education Academic Group, Nanyang Technological University
  • Wang Jing-Yuan
    Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University

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  • Bioconversion of Styrene to Poly(hydroxyalkanoate) (PHA) by the New Bacterial Strain Pseudomonas putida NBUS12

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Abstract

Styrene is a toxic pollutant commonly found in waste effluents from plastic processing industries. We herein identified and characterized microorganisms for bioconversion of the organic eco-pollutant styrene into a valuable biopolymer medium-chain-length poly(hydroxyalkanoate) (mcl-PHA). Twelve newly-isolated styrene-degrading Pseudomonads were obtained and partial phaC genes were detected by PCR in these isolates. These isolates assimilated styrene to produce mcl-PHA, forming PHA contents between 0.05±0.00 and 23.10±3.25% cell dry mass (% CDM). The best-performing isolate was identified as Pseudomonas putida NBUS12. A genetic analysis of 16S rDNA and phaZ genes revealed P. putida NBUS12 as a genetically-distinct strain from existing phenotypically-similar bacterial strains. This bacterium achieved a final biomass of 1.28±0.10 g L−1 and PHA content of 32.49±2.40% CDM. The extracted polymer was mainly comprised of 3-hydroxyhexanoate (C6 ), 3-hydroxyoctanoate (C8 ), 3-hydroxydecanoate (C10 ), 3-hydroxydodecanoate (C12 ), and 3-hydroxytetradecanoate (C14 ) monomers at a ratio of 2:42:1257:17:1. These results collectively suggested that P. putida NBUS12 is a promising candidate for the biotechnological conversion of styrene into mcl-PHA.

Journal

  • Microbes and Environments

    Microbes and Environments 30 (1), 76-85, 2015

    Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

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