Rapid In Situ Enumeration of Physiologically Active Bacteria in River Waters using Fluorescent Probes.
-
- YAMAGUCHI NOBUYASU
- Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University
-
- KENZAKA TAKEHIKO
- Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University
-
- NASU MASAO
- Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University
書誌事項
- タイトル別名
-
- Rapid In Situ Enumeration of Physiologi
この論文をさがす
抄録
In situ enumeration methods utilizing fluorescent probes were used to estimate the number of physiologically active bacteria in river water. Two fluorogenic compounds, 5-cyano-2, 3-ditolyl tetrazolium chloride (CTC) and 6-carboxy fluorescein diacetate (6CFDA), were chosen for direct epifluorescent microscopic detection of active bacteria. CTC is a soluble redox indicator which is reduced by respiring bacteria to fluorescent CTC-formazan crystals. 6CFDA is hydrolyzed by nonspecific esterases to produce the fluorescent compound, 6-carboxy fluorescein. Estimates of the number of active bacteria identified by these fluorescent probes were compared with those obtained with the plate count method. Bacteria with respiratory activity, as determined by CTC reduction, accounted for approximately 10% of the total bacteria at oligotrophic sites and 15 to 20% at eutrophic sites. These values exceeded those obtained with the plate count method. Estimates of enzymatically active bacteria, as determined by 6CFDA hydrolysis, were also higher than those obtained with the plate count method, constituting 40 to 50% of the total bacteria at both oligotrophic and eutrophic sites. These results indicate that some non-culturable bacteria retain physiologic activity and may play an important role in the ecosystem.
収録刊行物
-
- Microbes and environments
-
Microbes and environments 12 (1), 1-8, 1997
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679323374336
-
- NII論文ID
- 110001272793
-
- NII書誌ID
- AA11173196
-
- ISSN
- 13474405
- 13426311
- http://id.crossref.org/issn/13426311
-
- NDL書誌ID
- 4195085
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可