A New Fractionation and Recovery Method of Viral Genomes Based on Nucleic Acid Composition and Structure Using Tandem Column Chromatography

DOI Web Site PubMed 被引用文献12件 参考文献23件 オープンアクセス
  • Urayama Syun-ichi
    Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
  • Yoshida-Takashima Yukari
    Department of Subsurface Geobiological Analysis and Research, JAMSTEC
  • Yoshida Mitsuhiro
    Department of Subsurface Geobiological Analysis and Research, JAMSTEC
  • Tomaru Yuji
    National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency
  • Moriyama Hiromitsu
    Laboratory of Molecular and Cellular Biology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology
  • Takai Ken
    Department of Subsurface Geobiological Analysis and Research, JAMSTEC
  • Nunoura Takuro
    Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

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Metagenomic studies have revealed the unexplored diversity of the environmental virosphere. However, most studies are biased towards specific types of viral genomes due to the absence of universal methods to access all viral genome types. In the present study, we established a novel system to efficiently separate single- and double-stranded DNA/RNA viral genomes using hydroxyapatite and cellulose chromatography. This method will allow us to quantitatively and simultaneously access four types of viral genomes and will provide important clues to further understand previously unexplored environmental viral populations and obtain potentially unbiased libraries from environmental viral communities.

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