Availability of the Embryo of Semisulcospira libertina (Prosobranchia : Pleuroceridae) as a Laboratory Animal

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  • カワニナ胚の実験動物としての利用可能性
  • カワニナ ハイ ノ ジッケン ドウブツ トシテノ リヨウ カノウセイ

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Abstract

In order to examine the availability of embryos of Semisulcospira libertina as a laboratory animal, the optinum season for obtaining early embryos (fertilized egg to gastrula) from the brood pouch, optimum cultured temperature and optimum developmental stages were investigated with culture experiment. Early embryos (fertilized egg to gastrula) were continuously observed in the brood pouch from May to October. Embryos obtained each month were cultured under three different temperature conditions, by taking habitat water temperature into consideration. Embryos have well developed at 28℃ (July and August) and 25℃ (June and September), but not well at 18℃ (May and October). 28℃ was considered to be the highest temperature observed under natural conditions, and 25℃ would be the normal water temperature during the main ovulation season. Therefore, experiments were repeated at a constant temperature of 25℃. Embryos obtained in June, July, September and October developed well until the veliger stage. It was concluded that embryos developed beyond the morula stage obtained from June to October are highly suitable as materials for culture experiment at 25° or 28℃.

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