Analysis of Intestinal Microbiota Using T-RFLP

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  • T-RFLP法による腸内細菌叢の解析

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The molecular tool targeting the 16S ribosomal RNA gene is a powerful tool for elucidating the structure and function of bacterial communities. Here we give an outline of T-RFLP and its related techniques, mainly our devised one, as well as research findings that have been obtained using the T-RFLP. Moreover, we present research findings obtained using T-RFLP that are consistent with those obtained using next generation sequencers that have recently come into widespread use. We also discuss the future uses of the T-RFLP.<br>

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