Progress and Present Status of Methods for the Analysis of Intestinal Microbiota

DOI 79 References
  • FUJISAWA Tomohiko
    Department of Food Hygiene, Faculty of Applied Life Science, Nippon Veterinary and Life Science University
  • OHASHI Yuji
    Department of Food Hygiene, Faculty of Applied Life Science, Nippon Veterinary and Life Science University

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  • 腸内細菌検索法の変遷と現状
  • ―培養法からメタゲノム解析まで―
  • —From Cultivation to Metagenome Analysis—

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Many different kinds of bacteria are normally found in the intestines of healthy humans and animals. To study the ecology and function of these intestinal bacteria, the culture method is the basic technique. Suitable agar plates such as non-selective agar plates (BL agar and EG agar) and several selective agar plates have been developed. Furthermore, the roll-tube, glove box, and plate-in-bottle methods have also been developed for the cultivation of fastidious anaerobes which predominantly colonize the intestine. In addition, genomic analyse such as the FISH method, the PCR method, the clone-library method, the DGGE/TGGE methods, the T-RFLP method and metagenome analysis have been used for the investigation of intestinal microbiota in recent years. This paper summarizes the progress and present status of the methods of analysis for intestinal microbiota. <br>

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