Separation of Phospholipids from Hen Egg Yolk by High-Speed Countercurrent Chromatography

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  • SHIBUSAWA Yoichi
    Division of Structural Biology and Analytical Science, Tokyo University of Pharmacy and Life Science
  • TAKEUCHI Naoko
    Division of Structural Biology and Analytical Science, Tokyo University of Pharmacy and Life Science
  • SHINDO Heisaburo
    Division of Structural Biology and Analytical Science, Tokyo University of Pharmacy and Life Science
  • ITO Yoichiro
    Laboratory of Biophysical Chemistry, National Heart Lung, and Blood Institue, National Institutes of Health

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Other Title
  • 高速向流クロマトグラフィーによる鶏卵卵黄中のリン脂質の分離
  • コウソク コウリュウ クロマトグラフィー ニ ヨル ケイラン ランオウ チュウ ノ リン シシツ ノ ブンリ

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Description

Phospholipids were fractionated by high-speed counter-current chromatography in a one-step operation from about 0.75 g of hen egg yolk using a type J-multilayer coil planet centrifuge. The partition coefficients values of phospholipids were measured in several two or three-solvent systems. The separation of phospholipids was performed with a two-phase solvent system composed of chloroform/n -heptane/n -butanol/methanol/60% acetic acid (2 : 3 : 2 : 3 : 5) by eluting the lower phase at a flow-rate of 1.0 ml/min. The phospholipids in each fraction were characterized by thin-layer chromatography.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 54 (12), 1149-1154, 2005

    The Japan Society for Analytical Chemistry

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