Preparation of Monoclonal Antibody for Pesticide and Mycotoxin and Its Application to Food Analysis

DOI Web Site Web Site 2 Citations 40 References

Bibliographic Information

Other Title
  • 農薬やカビ毒に対するモノクローナル抗体の調製と食品分析技術への応用
  • ノウヤク ヤ カビ ドク ニ タイスル モノクローナル コウタイ ノ チョウセイ ト ショクヒン ブンセキ ギジュツ エ ノ オウヨウ

Search this article

Abstract

To analyze low-molecular compounds, such as pesticides and mycotoxin in food samples, an improved method of antibody preparation and determination methods using antibodies were examined. These methods were evaluated by applying them to real samples and by marketing them as commercially available products. Monoclonal antibodies (MoAbs) corresponding to required specifications in the field were necessary to develop determination methods with high reliability. We have designed the optimally modified constitution of low molecular compounds that successfully give immunogenicity to compounds, and prepared targeted MoAbs among many candidates by a unique screening method. Using the specified MoAbs, direct competitive ELISA (enzyme-linked immunosorbent assay) was developed for pesticide analysis, and an immuno-affinity column was developed for mycotoxin analysis. ELISA and the column have been commercialized as test kits and pre-packed columns, respectively, and are being actively used for food analysis.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 60 (5), 389-398, 2011

    The Japan Society for Analytical Chemistry

Citations (2)*help

See more

References(40)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top