Determination of glycation on diabetic erythrocytes by electromagnetophoresis

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  • NOZAKI Osamu
    Department of Clinical Laboratory Medicine, Kinki University School of Medicine
  • MUNESE Motonori
    Chemco Scientific Corporation Limited
  • KAWAMOTO Hiroko
    Department of Biological Regulation, School of Health Science, Faculty of Medicine, Tottori University

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Other Title
  • 電磁気泳動法による糖尿病赤血球膜糖化度測定
  • デンジキ エイドウホウ ニ ヨル トウニョウビョウ セッケッキュウ マク トウカド ソクテイ

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Abstract

A novel determination method for assaying glycation on erythrocytes by electromagnetophoresis was developed in this study. The prediction of angiopathy is necessary for treating diabetic patients, because the major cause of death of diabetic patients (type 2) is due to angiopathy. The levels of the fasted blood glucose and glycated hemoglobin A1c (HbA1c) are assayed for daily laboratory tests, but do not reveal the damage to the cells or vessels of diabetic patients. The aim of this study was to develop a novel method involving the electromagnetophoresis of diabetic erythrocytes. The method consisted of two steps. One was to bind boric acid-induced magnetic beads to a glycated membrane of erythrocytes; the second was to separate the complex of the erythrocytes and the magnetic beads by using an electric force for migrating the complex and a magnetic force for restricting the migration of the complex. We synthesized boric acid-introduced magnetic beads for this study. We chose 300 Gauss for the external magnetic force. A comparative study of the migration of erythrocytes by electrophoresis and electromagnetophoresis revealed a shorter migration of the erythrocytes by electromagnetophoresis than by electrophoresis, because the electrophoresis of erythrocytes reacted to the surface charge, and the electromagnetophoresis surface glycation of the erythrocytes. The electromagnetophoresis of the erythrocytes with values of HbA1c ranging from 5.4, 7.7, 8.6 and 9.3% resulted in a shorter migration of the erythrocytes in corporation to increase of the values of HbA1c. This meant that the method by elelctromagnetophoresis was successful for assaying glycation on erythrocytes, and will be a possible method for assessing the diabetic disturbance on cells.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 53 (2), 85-90, 2004

    The Japan Society for Analytical Chemistry

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