Space-Time Imaging of Bioluminescence from a Single Colony of Luminous Bacterium

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  • KARATANI Hajime
    Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology
  • HEGURI Atsushi
    Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology
  • HIRAYAMA Satoshi
    Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology
  • MATSUMURA-INOUE Takeko
    Minerva Light Lab.

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Other Title
  • 発光微生物単一コロニーの生物発光時空イメージング
  • ハッコウ ビセイブツ タンイツ コロニー ノ セイブツ ハッコウ ジクウ イメージング

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Abstract

We performed a space-time imaging analysis of a single colony of marine luminous bacterium using a fluorescent microscope connected to a real-color CCD camera. The bioluminescence and bright-field imaging showed that a glowing ring occurred close to the frontline of the growing colony, and that the ring spread out regularly while retaining a concentric ring shape. The glowing ring faded out when the colony terminated its growth. Furthermore, it was found that either aeration or O2-supply triggered a new glowing ring on the dim inside of the colony, and that the newly triggered ring phases out in the absence of aeration or O2-supply. Such a flickering pattern was confirmed to repetitively occur on the same concentric circle, corresponding to the presence and absence of aeration or O2-supply. From these findings, it is postulated that a growing single colony possesses a certain periodic property.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 56 (1), 43-46, 2007

    The Japan Society for Analytical Chemistry

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