Simple Pretreatment Procedure and Automated Immunoassay System for Detection of PCBs in Transformer Oil
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- OHMURA Naoya
- Central Research Institute of Electric Power Industry, Environmental Science Research Laboratory
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- GLASS Thomas R.
- Central Research Institute of Electric Power Industry, Environmental Science Research Laboratory
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- SASAKI Kazuhiro
- Central Research Institute of Electric Power Industry, Environmental Science Research Laboratory
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- JOH Takashi
- Electric Power Engineering Systems Co., Ltd.
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- TAEMI Yukihiro
- Electric Power Engineering Systems Co., Ltd.
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- YOKOBORI Naoyuki
- Sumika Chemical Analysis Service, LTD., Ehime Laboratory
Bibliographic Information
- Other Title
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- 簡易な前処理と自動装置を用いる絶縁油中のポリ塩化ビフェニルのイムノアッセイ
- カンイ ナ マエ ショリ ト ジドウ ソウチ オ モチイル ゼツエンユチュウ ノ ポリ エンカ ビフェニル ノ イムノアッセイ
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Abstract
A simple and rapid preparation procedure of PCBs in transformer oil in conjunction with an immunoassay using an automated instrument for detecting PCBs is described. The joint column is used to prepare PCBs. The oil sample is introduced into a primary diatomite column containing fuming sulfuric acid and sulfuric acid to degrade the hydrocarbons, besides PCBs; the resulting hydrocarbons are removed by a secondary silica column. PCBs in the elusion are then extracted by dimethyl sulfoxide for an immunoassay. This preparation procedure can be performed within 30 min., and the recovery ratio of PCBs is estimated to be in the range from 40% to 60%. PCBs can be detected with an automated immunoassay instrument after mixing the dimethyl sulfoxide extract at 1%(v/v) with an anti-PCB antibody solution. The assay is completed within 8 min. The dynamic range is, respectively, from 0.3 ppm to 18 ppm for Kanechlor 300, from 0.2 ppm to 0.7 ppm for Kanechlor 400, and from 0.1 ppm to 1.5 ppm for Kanechlor 500 and 600. This assay also enables one to detect PCBs as a total in fifteen oil samples in the range from 0.1 ppm to 1.8 ppm with a good correlation with the analysis using a high-resolution mass spectrometer.<br>
Journal
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- BUNSEKI KAGAKU
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BUNSEKI KAGAKU 55 (1), 1-7, 2006
The Japan Society for Analytical Chemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282679330683776
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- NII Article ID
- 110002973242
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- NII Book ID
- AN00222633
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- COI
- 1:CAS:528:DC%2BD28XmvVamtA%3D%3D
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- NDL BIB ID
- 7792988
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- ISSN
- 05251931
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- Abstract License Flag
- Disallowed