Microscopic Spectrophotometry Using Confocal Laser Scanning Microscope

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  • Matsumoto Tomoko
    Technical Center Faculty of Engineering, University of Miyazaki
  • Shiraki Ryuichi
    Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki
  • Matsumoto Jin
    Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki
  • Yasuda Masahide
    Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki
  • Shiragami Tsutomu
    Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki

Bibliographic Information

Other Title
  • 共焦点レーザー走査型顕微鏡を用いる顕微吸光度分析
  • キョウショウテン レーザー ソウサガタ ケンビキョウ オ モチイル ケンビ キュウコウド ブンセキ
Published
2008
Resource Type
journal article
DOI
  • 10.2116/bunsekikagaku.57.819
Publisher
The Japan Society for Analytical Chemistry

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Description

In order to elucidate the applicable range and accuracy of absorption spectrophotometry in the micro-region using a confocal laser scanning microscope (CLSM), micro-silica gel beads (2) immobilizing a given molar concentration (C1m) of dihydroxo(tetraphenylporphyrinato)- antimony(V) bromide (1) were prepared. The measurable area on the beads was determined to be a circle of 2.14 μm diameter by magnifications of an objective lens, the diameter of fiber core, and the diameter of a pinhole. Moreover, the range of the diameter of the beads and the spectral range were decided to be 4 μm∼350 μm and 488∼775 nm, respectively. Using a halogen lamp as a light source on CLSM, the molar concentrations (C2) of 1 in 2 were determined by absorption spectrophotometry. The value of C2 showed good agreement with C1m with 0.11∼0.73 of the standard deviation. Thus, absorption spectrophotometry in the micro-region will be a powerful tool for the quantitative analysis of transparent materials of micron-order.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 57 (10), 819-824, 2008

    The Japan Society for Analytical Chemistry

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