Rapid Identification of Hiochi Bacteria by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

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  • マトリックス支援レーザー脱離イオン化質量分析法による火落ち菌の迅速同定
  • マトリックス シエン レーザー ダツリ イオンカ シツリョウ ブンセキホウ ニ ヨル ヒオチキン ノ ジンソク ドウテイ

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Abstract

The rapid identification of the sake spoilage microorganisms (hiochi bacteria) by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using ribosomal proteins as biomarkers was developed. Ribosomal proteins isolated and purified from five representative hiochi bacteria (Lactobacillus fructivorans, L. hilgardii, L. paracasei subsp. paracasei, L. paracasei subsp. tolerans, and L. rhamnosus) as references were characterized by MALDI-MS. Among the peaks observed both from purified ribosomal protein samples and cell lysates in the range of m/z 6000∼12000, the 15 strongest peaks were selected for each reference hiochi bacteria to make a biomarker peak database. Since the m/z values of several biomarker peaks were identical to each bacteria, rapid discrimination and identification of hiochi bacteria could be performed at the species as well as the subspecies level. This method was then applied for the rapid identification of hiochi bacteria generated in a sake brewery. The m/z values of MALDI mass spectra of the hiochi bacteria sample suggested that this bacteria could be identified as L. paracasei subsp. paracasei or closely-related species.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 56 (12), 1071-1079, 2007

    The Japan Society for Analytical Chemistry

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