Flow-Injection Amperometric Determination of Urine Glucose Using Platinum Electrode Modified with Nafion<sup>®</sup> and Saccharide Substitued Polypyrrole

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  • Yasuzawa Mikito
    Department of Chemical Science and Technology, Institute of Technology and Science, The University of Tokushima
  • Kitahara Makoto
    Department of Chemical Science and Technology, Institute of Technology and Science, The University of Tokushima
  • Yabutani Tomoki
    Department of Chemical Science and Technology, Institute of Technology and Science, The University of Tokushima
  • Motonaka Junko
    Department of Chemical Science and Technology, Institute of Technology and Science, The University of Tokushima

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Other Title
  • ナフィオン<sup>®</sup>及び糖鎖を有するポリピロールを修飾した白金電極を用いる尿中グルコースのフローインジェクション/アンペロメトリック検出
  • ナフィオン及び糖鎖を有するポリピロールを修飾した白金電極を用いる尿中グルコースのフローインジェクション/アンペロメトリック検出
  • ナフィオン オヨビ トウサ オ ユウスル ポリピロール オ シュウショク シタ ハッキン デンキョク オ モチイル ニョウ チュウ グルコース ノ フローインジェクション アンペロメトリック ケンシュツ
  • Flow-Injection Amperometric Determination of Urine Glucose Using Platinum Electrode Modified with Nafion<sup>&reg;</sup> and Saccharide Substitued Polypyrrole

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Abstract

A flow-injection amperometric analysis system for the determination of glucose in human urine was analyzed. The glucose sample was mixed with glucose oxidase, and was introduced into the flow-injection system. Hydrogen peroxide produced by an enzymatic reaction was amperometrically detected by a platinum electrode modified with Nafion, and an electrochemically polymerized saccharide substituted polypyrrole. The influence of electroactive compounds such as ascorbic acid, uric acid, and acetaminophen to the glucose response observed in this system was negligible. A good linear relationship between the glucose concentration and the response current was obtained within a concentration of up to 22.4 mmol dm−3. The relative standard deviation for ten replicate analyses of 5.6 mmol dm−3 glucose in human urine was 7.4%.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 58 (11), 941-944, 2009

    The Japan Society for Analytical Chemistry

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