Determination of Phosphate in Seawater by Transient Isotachophoresis/Capillary Zone Electrophoresis with Suppressed Electroosmotic Flow

DOI NDL Digital Collections Web Site Web Site 2 Citations 69 References

Bibliographic Information

Other Title
  • 電気浸透流を抑制した一時的等速電気泳動／キャピラリーゾーン電気泳動法による海水中のリン酸イオンの定量
  • デンキ シントウリュウ オ ヨクセイ シタ イチジテキ トウソク デンキ エイドウ キャピラリー ゾーン デンキ エイドウホウ ニ ヨル カイスイチュウ ノ リンサン イオン ノ テイリョウ

Search this article

Description

We developed capillary zone electrophoresis (CZE) with indirect UV detection for the determination of phosphate in seawater using transient isotachophoresis (TITP) as an on-line concentration procedure. As a high-UV-absorbing compound in a background electrolyte (BGE), 2,6-pyridinedicarboxylic acid (PDC) was used. Hydroxypropyl methylcellulose (HPMC) was added to the BGE to suppress electroosmotic flow (EOF) and the dispersion of an analyte zone for the purpose of maximizing the concentration effect by TITP. Several analytical conditions were examined to separate low concentrations of phosphate from high concentrations of chloride and sulfate in seawater and to detect phosphate with high sensitivity. The following optimum conditions were established: BGE, 5 mmol/l PDC containing 0.01% HPMC adjusted to pH 3.5 with 1 mol/l sodium hydroxide; wavelength for detection, 200 nm; vacuum (5 in. Hg) injection period of sample, 1 σ (ca. 21 nl); terminating ion solution, 500 mmol/l 2-(N -morpholino)ethanesulfonic acid (MES) adjusted to pH 4.0 with 1 mol/l sodium hydroxide; vacuum injection period of the terminating ion solution, 30 σ (ca. 630 nl); applied voltage, 15 kV with the sample inlet side as the cathode. Phosphate in seawater samples was detected for approximately 15 min. The limit of detection (LOD) for phosphate was 32 μg/l (PO43−-P) at a signal-to-noise ratio of three. The respective values of the relative standard deviation (RSD) of the peak area, peak height, and migration time for phosphate were 2.1, 2.3, and 0.81%. The proposed method determined phosphate in surface seawater samples.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 55 (9), 627-634, 2006

    The Japan Society for Analytical Chemistry

Citations (2)*help

See more

References(69)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top