Sample Preparation Method for Simultaneous Determination of the Content and Molecular Weight of Cationic Polymers in Hair Shampoo by Size-Exclusion Chromatography

SUGIYAMA Junichi, HANIHARA Hiroyuki, TANAKA Kosuke, IGARASHI Akinori, FUJIWARA Masami

doi:10.2116/bunsekikagaku.55.95

Bibliographic Information

Other Title

サイズ排除クロマトグラフィーによるヘアシャンプー中のカチオン性高分子の定量と分子量測定のための試料溶液調製法
サイズハイジョクロマトグラフィーニヨルヘアシャンプーチュウノカチオンセイコウブンシノテイリョウトブンシリョウソクテイノタメノシリョウヨウエキチョウセイホウ

Search this article

Abstract

In previous paper, we established an accurate analyzing method by combining a size-exclusion chromatography (SEC) with multi-angle laser light scattering (MALLS) and a refractive index detector (RI) for determining the amount and absolute molecular weight of the cationic cellulose derivative {cellulose, 2-hydroxyethyl 2-[2-hydroxy-3-(trimethylammonio)propoxy]ethyl 2-hydroxy-3-(trimethylammonio)propylether,chloride} (CC), which is the most popular cationic polymer used in hair shampoo. However, an appropriate sample-preparation method for the analysis has not been established due to the lack of separation and dissolution procedures of the CC from the shampoo. In this work, we investigated a sample-preparation method in which the CC is efficiently separated from the shampoo without its molecular deterioration. We found that the CC is clearly solubilized by the SEC mobile phase (0.3 mol/l NaClO₄ aqueous solution) as long as the CC is separated from the shampoo, because the tetrahydrofuran insoluble matter is immediately diluted by the mobile phase before drying out. In the SEC measurement, the CC from the model shampoo, the recovery rate and the reproducibility were sufficient. Moreover, no deterioration of the CC through the sample preparation was confirmed from a comparison between the molecular weight of the CC separated from the shampoo and that of the standard CC.<br>

Journal

BUNSEKI KAGAKU

BUNSEKI KAGAKU 55 (2), 95-100, 2006

The Japan Society for Analytical Chemistry

Keywords

Details 詳細情報について

Export