カルボン酸の蛍光プレラベル化剤としてのアントラセン誘導体の比較検討(<特集>バイオアナリティカルケミストリー)

DOI NDLデジタルコレクション Web Site Web Site 被引用文献2件

書誌事項

タイトル別名
  • Comparative investigation of anthracene derivatives as fluorescen pre-labeling agents for carboxylic acids.
  • カルボン酸の蛍光プレラベル化剤としてのアントラセン誘導体の比較検討
  • カルボンサン ノ ケイコウ プレラベルカザイ トシテノ アントラセン ユウドウ

この論文をさがす

抄録

Three anthracene derivatives {1-bromomethylanthracene (1-BrMA), 9-bromomethylanthracene (9-BrMA) and 9-chloroacetylanthracene (9-ClAA)} were investigated for their abilities as fluorescent pre-labeling agents for HPLC analysis of saturated aliphatic carboxylic acids. After various conditions such as reaction temperature, reaction time, and reagent concentration were examined, the derivatization procedures were established for ten saturated fatty acids (C4, C6, C8, C10, C12, C14, C1618 and C20). In the next step, separation conditions of the derivatives were studied for a reversed phase column, WAKOSIL 5C-8, with gradient elution using 80100% (v/v) aqueous methanol. According to the conditions established, it was shown that all the fluorescent esters produced by the derivatization of the acids with the three reagents could be separated within ca. 25 min. The detection limits for caproic acid were 100folds lower by using 1-BrMA or 9-BrMA {100 fmol (S/N≥3)} than by 9-ClAA (10 pmol).

収録刊行物

  • 分析化学

    分析化学 38 (11), 558-561, 1989

    公益社団法人 日本分析化学会

被引用文献 (2)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ