Determination of Iodide Ion in Iodine-Enriched Egg by Solid-Phase Extraction/Reversed-Phase High-Performance Liquid Chromatography

Miyashita Masahiro, Seyama Yoshiyuki, Yokoyama Jiro

doi:10.2116/bunsekikagaku.57.747

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固相抽出／逆相高速液体クロマトグラフィーによるヨード強化卵中のヨウ化物イオンの定量
コソウチュウシュツギャクソウコウソクエキタイクロマトグラフィーニヨルヨードキョウカランチュウノヨウカブツイオンノテイリョウ

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Abstract

We describe a simple and sensitive method for the determination of iodie (I⁻) in iodine-enriched eggs (IEs) by high-performance liquid chromatography (HPLC). I⁻ in a 0.05 g portion of egg yolk and 0.5 g of egg white of IEs were extracted by a solid-phase extraction (SPE) procedure with a cation-exchange and anion-exchange SPE cartridges (Oasis MCX and Oasis WAX). The I⁻ extracts were evaporated to dryness under a vacuum, and the residues were reconstituted in an HPLC mobile phase to prepare 1 mL of analytical sample solutions. A 10 μL aliquot of each sample solution was analyzed by ion-pair reversed-phase HPLC with a UV detector at 225 nm. The method gives good sample clean-up. The relative standard deviation for the determination of I⁻, n=4, in IE yolk and IE white were 0.8 and 5.5%, respectively. The recoveries of 50 μg of I⁻ spiked per g of IE yolk and 2.5 μg of I⁻ spiked per g of IE white were 92.4 and 96.8%, respectively. The detection limits for I⁻, based on S/N=3, were 0.76 μg per g of IE yolk and 0.08 μg per g of IE white. We applied successfully the method for the determination of I⁻ in commercial IEs.

Journal

BUNSEKI KAGAKU

BUNSEKI KAGAKU 57 (9), 747-754, 2008

The Japan Society for Analytical Chemistry

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