Visual Microplate Method with Color Changing Reactions used in Titrations

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  • MANAKA Atsushi
    Advanced Engineering Faculty, Toyama national college of technology
  • ISHIBASHI Manami
    Department of Biomolecular Functional Engineering, College of Engineering, Ibaraki University
  • NAKAYAMA Aya
    Chemical and Biochemical Engineering, Toyama national college of technology
  • NODA Miho
    Chemical and Biochemical Engineering, Toyama national college of technology
  • KATO Takeshi
    Ibaraki Prefectural Industrial Technology Center
  • IGARASHI Shukuro
    Department of Biomolecular Functional Engineering, College of Engineering, Ibaraki University

Bibliographic Information

Other Title
  • 滴定法の変色反応を用いる目視‐マイクロプレート法とその評価
  • 滴定法の変色反応を用いる目視-マイクロプレート法とその評価
  • テキテイホウ ノ ヘンショク ハンノウ オ モチイル モクシ マイクロ プレートホウ ト ソノ ヒョウカ

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Abstract

Visual analysis by counting to number of changing color on a microplate was examined using the reaction used for several titration analysis (neutralization titration, oxidation-reduction titration and chelatometric titration). After the addition of a sample to the microplate to which the several concentrations of a standard solution were added with the indicator at the each rows, the numbers of discoloring were changed with the sample concentration. As a result, the numbers of discoloration have changed according to each sample concentration even though the reaction of which titration was used. Moreover, from the result of a comparison with the proposed method and commercial colorimetry, it was able to be confirmed that this method was an effective technique as the reliability of the analytical value.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 60 (4), 363-366, 2011

    The Japan Society for Analytical Chemistry

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