Simple SNP Genotyping of Human Earwax-Type Gene ABCC11 for Genetic Education

DOI DOI IR Web Site Web Site 9 References

Bibliographic Information

Other Title
  • 遺伝子診断教育のための簡便な耳垢型遺伝子多型解析法
  • イデンシ シンダン キョウイク ノ タメ ノ カンベン ナ ミミアカガタ イデンシ タケイ カイセキホウ

Search this article

Abstract

Recently, progress in molecular biology is being made in the fields of science, medicine, and pharmacy. Therefore, the majority of students graduating from pharmaceutical university departments will be required to have knowledge of molecular biology techniques. However, it is difficult to include such experiments in the curriculum for students at general high schools and universities because of safety concerns, the expensive materials and experimental equipment and time consumed. Therefore, this report introduces the convenient experimental materials on human DNA polymorphism for the genotyping of the earwax type gene ABCC11 on the chromosome 16 using a PCR-RFLP method. This experiment enables each student to handle DNA safely. The total time required for the experiments is less than 4 hours. The PCR-RFLP experiment proposed here is a suitable genotyping method to acquire molecular biological knowledge and techniques.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 59 (7), 613-617, 2010

    The Japan Society for Analytical Chemistry

References(9)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top