Redox color reagents for the assay of hydroperoxides
-
- KASAHARA Shizuka
- Wako Pure Chemical Industries, Ltd., Osaka Research Laboratory
-
- YAMANISHI Kazuhiko
- Wako Pure Chemical Industries, Ltd., Osaka Research Laboratory
-
- HANADA Toshiro
- Wako Pure Chemical Industries, Ltd., Osaka Research Laboratory
-
- TOKUDA Kuniaki
- Wako Pure Chemical Industries, Ltd., Osaka Research Laboratory
-
- MISU Kiyoshi
- Wako Pure Chemical Industries, Ltd., Osaka Research Laboratory
-
- SUGANO Masahiko
- Wako Pure Chemical Industries, Ltd., Osaka Research Laboratory
Bibliographic Information
- Other Title
-
- 過酸化物質測定用酸化還元呈色試薬
- カサンカ ブッシツ ソクテイヨウ サンカ カンゲン テイショク シヤク
Search this article
Abstract
Various hydroperoxides, such as cumen hydroperoxide, t-butyl hydroperoxide and ethyloleate hydroperoxide, react with new color reagents containing 4-aminoantipyrine (4-AA) or 3-methyl-2-benzothiazolinone hydrazone (MBTH), N, N-dialkylaniline derivatives and iron(II) ion, in acidic medium to yield quinoneimine type chromogens {λmax: (530601)nm}. From the results obtained by various combinations of reagents, the combinations of N-ethyl-N-(β-hydroxyethyl)-3-methylaniline and 4-AA (Reagent A) or MBTH (Reagent B) were recommended. The reagents and the assay procedure are as follows: Reagent A (or B) was prepared by dissolving 8.4 m mol of N-ethyl-N-(β-hydroxyethyl)-3-methylaniline, 1.5 mmol of 4-AA (or MBTH) and 50 mg of ferrous ammonium sulfate in 11 of 0.05 M acetate buffer (pH 4.0). A sample containing (40100)nmol of hydroperoxide was mixed with 4 ml of 2-propanol and centrifuged. One ml of reagent A was then added to 3 ml of the supernatant solution, and the mixture was allowed to stand for 60 min at 37°C. The developed colored solution was measured spectrophotometrically. When reagent B was used, 1-propanol was used instead of 2-propanol.
Journal
-
- BUNSEKI KAGAKU
-
BUNSEKI KAGAKU 28 (7), 405-410, 1979
The Japan Society for Analytical Chemistry
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282679331979008
-
- NII Article ID
- 110002910431
-
- NII Book ID
- AN00222633
-
- COI
- 1:CAS:528:DyaE1MXlvVKisL0%3D
-
- NDL BIB ID
- 2058851
-
- ISSN
- 05251931
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
-
- Abstract License Flag
- Disallowed