Determination of Urinary Protein with o-Sulfophenylfluorone-Niobium(V) Complex

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  • ｏ‐スルホフェニルフルオロン‐ニオブ（Ｖ）錯体を用いる尿タンパク質の定量
  • o-スルホフェニルフルオロン-ニオブ(5)錯体を用いる尿タンパク質の定量
  • o スルホフェニルフルオロン ニオブ 5 サクタイ オ モチイル ニョウ タンパクシツ ノ テイリョウ

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Abstract

A spectrophotometric method was established for the determination of urinary protein with an o-sulfophenylfluorone-niobium(V) {SPF-Nb(V)} complex. This method is about 1.2-times more sensitive than the pyrogallol red-molybdenum(VI) method (PR method), and can determine trace amount of urinary protein. There was good for agreement between the results obtained for urinary protein by this method and those obtained by PR method (r = 0.914). The binding process between the {SPF-Nb(V)} complex and HSA was studied by determining the thermodynamic parameters. Moreover, upon application of this method to a protein assay kit, a mixed reagent was constructed and a simple sensitive method was developed for a visual detection of protein. The proposed method should be useful for a simple, rapid and sensitive determinations of urinary protein.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 57 (8), 637-642, 2008

    The Japan Society for Analytical Chemistry

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