Flow-injection analytical system for the evaluation of antioxidative activity

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  • 抗酸化活性評価のためのフローインジェクション分析システム
  • コウサンカ カッセイ ヒョウカ ノ タメ ノ フローインジェクション ブンセキ システム

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Abstract

Reactive oxygen species (ROSs) and various free radicals are known to cause serious diseases, such as cancer and arteriosclerosis, and to accelerate aging. In order to protect our health from oxidative stress, active research has been carried out on screening antioxidants that can quench or scavenge ROSs and free radicals formed in our body. In the present paper, several novel flow-injection analysis (FIA) systems are discribed for estimating the antioxidative activity rapidly and conveniently, as follows: (1) spectrophotometric and chemiluminometric FIA for estimating the superoxide dismutase (SOD) and superoxide anion-scavenging activity (SOSA), (2) amperometric FIA for monitoring the catalase activity, which can decompose hydrogen peroxide, (3) FIA for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity using electron spin resonance (ESR) as a detector, and (4) spectrophtometric FIA for 2,2'-azino-bis(3-benzthiazoline-6-sulfonic acid)(ABTS) radical-scavenging acitivity. Applications to practical samples, such as biochemical materials and foods, are also described.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 53 (4), 221-231, 2004

    The Japan Society for Analytical Chemistry

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