Semi-Micro Column HPLC of Three Oxicam Non-Steroidal Anti-Inflammatory Drugs in Human Blood

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  • NAKAMURA Akiko
    Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University
  • NAKASHIMA M. N.
    Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University
  • WADA Mitsuhiro
    Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University
  • NAKASHIMA Kenichiro
    Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University

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Other Title
  • ヒト血中の３種のオキシカム系非ステロイド性抗炎症薬のセミミクロカラム高速液体クロマトグラフィー
  • ヒト ケッチュウ ノ 3シュ ノ オキシカムケイ ヒステロイドセイ コウエンショウヤク ノ セミミクロカラム コウソク エキタイ クロマトグラフィー

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Description

A simple and rapid semi-micro column high-performance liquid chromatographic (HPLC) method with UV detection was developed for the simultaneous detrmination of oxicam non-steroidal anti-inflammatory drugs in human blood samples. Oxicams including isoxicam as an internal standard were extracted from buffered plasma samples (pH 3) with dichloromethane, and the resulting extracts were subjected to HPLC analysis. The separation was performed with a C18 reversed-phase semi-micro column (250 × 1.5 mm i.d., 5 μm) at 35°C. The mobile phase used was a mixture of acetonitrile-0.1 M acetate buffer (pH 5.0)-methanol, and the detection wavelength was set at 365 nm. Four oxicams were well-separated within 30 min without interference by the blood components. The detection limits of tenoxicam, piroxicam, and lornoxicam were 2.3, 4.2, 6.4 ng/ml in serum, and 2.7, 4.7, 9.3 ng/ml in plasma at a signal-to-noise ratio of 3, respectively. The method was applied to the determination of lornoxicam in the sera of patients.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 54 (9), 755-760, 2005

    The Japan Society for Analytical Chemistry

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