Determination of Adenine and Its Related Compounds Using Eosine and Silver(I)

NISHIMURA Michiko, NAKASHIMA Katsura, FUJIMOTO Tsuyoshi, YAMAGUCHI Takako, FUJITA Yoshikazu

doi:10.2116/bunsekikagaku.54.761

A spectrophotometric method for the determination of adenine and its related compounds based on an association complex between [silver-adenine] and Eosine was established. The standard procedure is as follows: The following components were mixed in a 10 ml volumetric flask: a solution containing of adenine, 0.8 ml of a 5.0 × 10⁻⁴ mol dm⁻³ silver(I) solution, 2.0 ml of the buffer solution, 1.0 ml of a 0.5% MC(1000∼1800 cps) solution and 0.8 ml of a 5.0 × 10⁻⁴ mol dm⁻³ Eosine solution. The mixture was diluted to 10 ml with water, transferred into a test tube, mixed well and kept at 60°C for 20 min. After cooling for 5 min in water, the absorbance of the resultant solution was measured at 560 nm against a reagent blank prepared under the same conditions. This method can be used to determine 0.1∼1.5 μg/ml adenine; the molar absorptivity is 1.1 × 10⁵ dm³ mol⁻¹ cm⁻¹ at 560 nm. This procedure is much more sensitive than the method using the Bratton-Marshall reagent. The relative standard deviation for 8 runs of 0.68 μg/ml adenine was 0.87%. No metal ion interfered in an equimolor ratio of adenine among the examined metal ions. A sensitive preconcentration method for the spectrophotometry of trace adenine that involves the filtration of an association complex on a membrane filter followed by its redissolution or by tristimulus colorimetry, is described. The thermodynamic parameters suggest that the colored complex formed in this reaction system is an association complex between [silver-adenine] and Eosine.<br>

Determination of Adenine and Its Related Compounds Using Eosine and Silver(I)

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