Enhancement in Separation Efficiency by Control in Electric Field on Pressurized-Flow Driven Capillary Electrochromatography

DOI IR NDL Digital Collections Web Site Web Site 1 Citations 48 References
  • Kitagawa Shinya
    Materials Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology

Bibliographic Information

Other Title
  • 圧力差流を用いる電気クロマトグラフィーにおける電場の制御による分離性能の向上
  • アツリョクサリュウ オ モチイル デンキ クロマトグラフィー ニ オケル デンバ ノ セイギョ ニ ヨル ブンリ セイノウ ノ コウジョウ

Search this article

Abstract

Pressurized-flow driven capillary electrochromatography (pCEC) is a hybrid separation method of liquid chromatography and capillary electrophoresis, in which an application of either positive or negative voltage, i.e., the electrophoretic migration of analytes toward either inlet or outlet, can be utilized to enhance the separation performance. By using intentional electrophoresis of analytes, an alteration of the separation selectivity and a reduction of the separation period through selective acceleration were achieved. The dual-gradient pCEC by varying the mobile phase composition, including a shift in the pH of the eluent and the applied voltage was effective to increase the separation performance. The combination of two different separation mechanisms of chromatography and electrophoresis in CEC was effective for comprehensive separation, and the separation efficiency was enhanced with an increase in the applied voltage. Furthermore, the electrophoresis of the analyte in a heterogeneous separation field under a gradient elution was effective to reduce the peak widths, i.e., zone sharpening.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 57 (12), 937-948, 2008

    The Japan Society for Analytical Chemistry

Citations (1)*help

See more

References(48)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top