Development of selective methods for the determination of small amounts of D-amino acids in mammals
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- HAMASE Kenji
- Graduate School of Pharmaceutical Sciences, Kyusyu University
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- ZAITSU Kiyoshi
- Graduate School of Pharmaceutical Sciences, Kyusyu University
Bibliographic Information
- Other Title
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- ほ乳類体内微量D‐アミノ酸の選択的分析法の開発
- 哺乳類体内微量D-アミノ酸の選択的分析法の開発
- ホニュウルイ タイナイ ビリョウ D アミノサン ノ センタクテキ ブンセキホウ ノ カイハツ
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Description
Sensitive and selective methods for the determination of small amounts of D-amino acids in mammalian tissues have been established using a two-step HPLC system with a reversed-phase column and a chiral column. A chiral derivatization method using o-phthalaldehyde and N-tert-butyloxycarbonyl-L-cysteine has also been reported for the simultaneous reversed-phase HPLC separation of D-aspartic acid, D-serine and D-alanine. Using these methods, the distribution and alteration of the amounts of D-alanine, D-leucine and D-proline in mammals have been clarified for the first time, and compared with those of D-aspartic acid and D-serine, which have already been reported for several mammalian tissues. The amounts of D-alanine, D-leucine and D-proline are much smaller than those of D-aspartic acid and D-serine, and the values are less than 1% of that of the L-form in most tissues. The amounts of D-alanine have been determined in 22 tissues of rats including the central nervous system and the periphery, and the highest value of 86.4 nmol/g wet tissue has been observed in the anterior pituitary gland. The amounts of D-alanine in this tissue increased until 6 weeks of age, and gradually decreased thereafter. The circadian changes of the D-alanine amounts in the anterior pituitary gland have also been revealed to show higher amounts during the light period. The distribution and alteration of the amounts of D-leucine and D-proline are totally different from those of D-alanine, indicating that these D-amino acids are strictly recognized in mammalian tissues.<br>
Journal
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- BUNSEKI KAGAKU
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BUNSEKI KAGAKU 53 (7), 677-690, 2004
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679332774144
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- NII Article ID
- 110002905412
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- NII Book ID
- AN00222633
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- COI
- 1:CAS:528:DC%2BD2cXlvVOmtbY%3D
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- NDL BIB ID
- 7033789
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- ISSN
- 05251931
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL Search
- Crossref
- NDL Digital Collections (NII-ELS)
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed