Flow Injection Analysis of Choline in Supplements Using Choline Oxidase Immobilized Enzyme Reactor Column

NAKANO Masahumi, YOSHIWARA Masaaki, SAKURAGAWA Akio

doi:10.2116/bunsekikagaku.56.765

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Other Title

コリンオキシダーゼ固定化酵素反応カラムを用いるサプリメント中のコリンのフローインジェクション分析
コリンオキシダーゼコテイカコウソハンノウカラムオモチイルサプリメントチュウノコリンノフローインジェクションブンセキ

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Description

A simple and sensitive flow injection analysis (FIA) system with immobilized choline oxidase (ChO) and peroxidase (POD) enzyme reactor columns for the determination of choline has been developed. ChO was immobilized onto chitosan as a support by cross-linking with glutaraldehyde by forming a Schiff base, and choline was determined with fluorescence detection. The FIA system with the immobilized enzyme reactor columns was tested for the parameters of the optimization procedure: carrier solution, flow rate and injected sample volume. After the optimization of the FIA parameters, the response time was about 3 (min), the linear calibration curve was obtained between 0.2 μmol dm⁻³ and 10 μmol dm⁻³of choline (r² = 0.999) and the detection limit was 0.1 μmol dm⁻³. After researching of the effects of coexisting substances by an additional recovery test, the FIA system was applied for a supplement sample and the relative standard deviations by repeated measurements (n = 5) were found to be within 0.3%. It was found that the FIA system can be applied to any samples without effecting of the coexisting substances.<br>

Journal

BUNSEKI KAGAKU

BUNSEKI KAGAKU 56 (9), 765-771, 2007

The Japan Society for Analytical Chemistry

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