Liquid chromatography using flow coulometry for anions with a hydrogen type cation exchange resin

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  • 電量検出型液体クロマトグラフィー　ＩＩ　　水素型陽イオン交換樹脂による陰イオンの電流検出型液体クロマトグラフィー
  • 水素型陽イオン交換樹脂による陰イオンの電量検出型液体クロマトグラフィー
  • ミズ ソケイ ヨウイオン コウカン ジュシ ニヨル インイオン ノ デンリョウ
  • 電量検出型液体クロマトグラフィーに関する研究(第2報)

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Description

A method for indirect determination of anion was investigated by liquid chromatography using a hydrogen type cation exchange column {resin : Hitachi-2613, (17±2) micron; column : 500×9 mm i. d.; eluent: water} and a flow coulometric detector. In order to determine indirectly the anion, the cation to be the counter ion of an anion was exchanged with hydrogen type cation exchange resin, and the hydrogen ion in column effluent reacted with p-quinone at the constant voltage of +0.45 V vs. Ag-AgI was determined by measuring coulomb in the reduction to hydroquinone. The retention volume (Vr) of the anions to form the strong acids by ion-exchange, such as chloride, nitrate, sulfate and dodecylbenzenesulfonate ions was about 12.5 ml. The Vr of the anions in the case of weak acids showed lower values than that of the strong acids, as follows; 13.0 ml for oxalate ion, 14.2 ml for phosphate ion, 14.5 ml for sulfite ion, 18.4 ml for floride ion, 19.5 ml for formate ion, 23.3 ml for acetate ion and 29.1 ml for carbonate ion, respectively.<BR>The linear relationship was obtained between the Vr of the anions and the primary dissociation constant (pK1) of the acids librated by ion exchange. The eluting process was considered as a partition chromatography based on ion exclusion. The calibration curve for sulfate ion was obtained with good linearity for the range of 5 to 1000 μg/ml, and the detection limit was 0.14μg/ml. The reproducibility on the chromatogram area of 100μg/ml sulfate ion was±0.69%.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 24 (4), 235-238, 1975

    The Japan Society for Analytical Chemistry

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