Application of 2-(2,3-naphthalimino)ethyl trifluoromethanesulphonate as a pre-column derivatization reagent for high-performance liquid chromatography in the determination of 3-hydroxybutyrate in aqueous solution
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- NAKAHARA Rie
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences
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- INAMURA Katsushi
- Department of Pharmacy, Toyama Rousai Hospital
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- WATANABE Gen
- Department of Pharmacy, Toyama Rousai Hospital
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- OHTA Shin
- Department of Pharmacy, Nagano Red Cross Hospital
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- SHIMADA Kenji
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences
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- YAMATO Susumu
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences
Bibliographic Information
- Other Title
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- 高速液体クロマトグラフィー用プレカラム誘導体化試薬2-(2,3-ナフタルイミノ)エチルトリフルオロメタンスルフォナートの水溶液中3-ヒドロキシ酪酸定量への応用
- コウソク エキタイ クロマトグラフィーヨウ プレカラム ユウドウタイカ シヤク 2 2 3 ナフタルイミノ エチルトリフルオロメタンスルフォナート ノ スイヨウエキ チュウ 3 ヒドロキシラクサン テイリョウ エ ノ オウヨウ
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Abstract
2-(2,3-Naphthalimino)ethyl trifluoromethanesulphonate (NE-OTf), as a pre-column derivatization reagent for high-performance liquid chromatography, has been tried for the determination of 3-hydroxybutyrate in aqueous solution. Thus far, NE-OTf has been inapplicable to short-chain carboxylic acids and an aqueous reaction system. 3-Hydroxybutyrate in aqueous solution reacted completely with an excess amount of NE-OTf in the presence of dimethyl sulfoxide, and N-ethyldiisopropylamine under a water-bath with sonic vibration setted at 45°C for 40 min. Under this condition, excess amounts of NE-OTf were also completely destroyed, and any chromatographic peaks due to by-products did not interfere with the determination of 3-hydroxybutyric acid ester derivative. The calibration curve of a 3-hydroxybutyrate standard solution was linear over the range of 0.02∼2.0 nmol for 10 μl injection, with a correlation coefficient of 0.9988. The detection limit (signal-to-noise ratio = 3) for 3-hydroxybutyrate was 2 pmol for 10 μl injection. By this method, NE-OTf was successfully applied to the determination of 3-hydroxybutyrate in aqueous solution.<br>
Journal
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- BUNSEKI KAGAKU
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BUNSEKI KAGAKU 53 (3), 161-165, 2004
The Japan Society for Analytical Chemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282679333158272
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- NII Article ID
- 110002905351
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- NII Book ID
- AN00222633
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- COI
- 1:CAS:528:DC%2BD2cXisF2rtL4%3D
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- NDL BIB ID
- 6889244
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- ISSN
- 05251931
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- Abstract License Flag
- Disallowed