The Optimum Recrystallization of Berberine Chloride and Its Quality Check by HPLC

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  • 塩化ベルベリンの再結晶条件の最適化及び高速液体クロマトグラフィーによる純度測定
  • エンカ ベルベリン ノ サイケッショウ ジョウケン ノ サイテキカ オヨビ コウソク エキタイ クロマトグラフィー ニ ヨル ジュンド ソクテイ

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Abstract

Berberine and palmatine are isoquinoline alkaloids contained in “oubaku”, Phellodendri Cortex, and their structures are similar. The Rf values of TLC and the retention times (HPLC) of these compounds are close. Their complete separation is difficult; a small quantity of palmatine chloride exists even in a commercial berberine chloride. Some crystals exist in the Student practice, from gel-type crystals (small needles) to big needles according to the volume of water. These phenomena suggest that a possibility exist to purify berberine chloride only by recrystallization. We recrystallized 3 times crude berberine (10 g, gained from the Student practice) from 140 ml of water per 1 g, and separated pure needles (neally 100%, 2.31 g) which were checked with HPLC.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 54 (1), 79-82, 2005

    The Japan Society for Analytical Chemistry

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