Elimination of Spectral Interferences for the Determination of Fe and Se in Biological Samples Using ICP-Ion Trap Mass Spectrometer

HORIGOME Jun, FURUTA Naoki

doi:10.2116/bunsekikagaku.54.373

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Other Title

誘導結合プラズマイオントラップ質量分析計を用いる生体試料中の鉄及びセレン測定時のスペクトル干渉の低減
ユウドウケツゴウプラズマイオントラップシツリョウブンセキケイオモチイルセイタイシリョウチュウノテツオヨビセレンソクテイジノスペクトルカンショウノテイゲン

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Description

An inductively coupled plasma mass spectrometer with an ion-trap mass spectrometer was used for the determination of Fe and Se in biological samples. The spectral interferences of Ar₂⁺, ³¹P₂¹⁶O⁺, ¹²C³⁵Cl₂⁺ and ³²S¹⁶O₃⁺ on ⁷⁸Se⁺, ⁸⁰Se⁺ and ⁸²Se⁺ were eliminated by optimizing the ion level and FNF (filtered noise field) function of 3DQMS (three dimensional quadrupole mass spectrometer). As a result of decreasing ⁴⁰Ar₂⁺, a linearity of 7 orders of magnitude from 100 ppt to 100 ppm and a limit of detection of 11 ppt were achieved at ⁸⁰Se. The spectral interferences of ⁴⁰Ar¹⁶O⁺, ⁴⁰Ca¹⁶O⁺, ⁷⁹Br¹H⁺ and ⁸¹Br¹H⁺ decreased by using the CID (collision induced dissociation) function of 3DQMS. The optimum conditions of CID obtained from the signal intensity and the isotopic ratio of Fe and Se were 0 V and 500 ms. Under the optimum conditions of the ion level, FNF and CID, Se in human urine standard materials (NIES CRM 18 and Seronorm 2524) was determined; the analytical results showed good agreement with reference values within the analytical error, and a recovery of 101% could be attained for Fe in human urine standard material (Seronorm 2524). The developed method enabled us to determine Se and Fe in biological samples by using the most abundant isotopes of ⁵⁶Fe and ⁸⁰Se.<br>

Journal

BUNSEKI KAGAKU

BUNSEKI KAGAKU 54 (5), 373-380, 2005

The Japan Society for Analytical Chemistry

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