Simultaneous Determination of Nitrite, Nitrate, and Ammonium Ions in Lake and River-Water Samples by Ion Chromatography

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  • Tanaka Masahito
    Cluster of Biotechnology and Chemistry Systems, Graduate School of Systems Engineering, Kinki University
  • Ohguni Hideto
    Cluster of Biotechnology and Chemistry Systems, Graduate School of Systems Engineering, Kinki University
  • Adachi Katsuhiko
    Department of Biotechnology and Chemistry, School of Engineering, Kinki University
  • Tsumura Tomoaki
    Department of Biotechnology and Chemistry, School of Engineering, Kinki University
  • Hirokawa Takeshi
    Department of Applied Chemistry, Graduate School of Engineering, Hiroshima University
  • Ito Kazuaki
    Cluster of Biotechnology and Chemistry Systems, Graduate School of Systems Engineering, Kinki University Department of Biotechnology and Chemistry, School of Engineering, Kinki University

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Other Title
  • イオンクロマトグラフィーによる湖水及び河川水中の亜硝酸イオン，硝酸イオン，アンモニウムイオンの同時定量
  • イオン クロマトグラフィー ニ ヨル コスイ オヨビ カセン スイチュウ ノ アショウサン イオン ショウサン イオン アンモニウムイオン ノ ドウジ テイリョウ

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Description

The simultaneous determination of inorganic nitrogen species (nitrite, nitrate, and ammonium ions) in lake and river-water samples was examined by ion chromatography. Nitrite and nitrate ions were separated on a monolith type of ODS columns equilibrated with DDAB (dilauryldimethylammonium bromide) using 0.5 M sodium chloride + 5 mM phosphate buffer (pH = 5.0) as the eluent and they were detected by UV at 225 nm. Whereas ammonium ion was detected at 710 nm as an indophenol blue derivative after the post-column reaction of ammonium in the presence of dichloroisocyanurate and 1-naphthol. Calibration curves of all ions showed good linearity (more than R2 = 0.998) in the range of 0∼10 mg/L. The concentration and pH of reagents, the length of reaction coil, and the flow rate of eluent were optimized. The detection limits (S/N = 3) of nitrite, nitrate, and ammonium ions were 0.2, 0.7, and 2.7 μg/L, respectively. Also, the repeatability (0.1 mg/L, n = 5) was below 0.15% (retention time), 1.13% (peak area), and 0.88% (peak height). The present method was applied to the simultaneous determination of inorganic nitrogen species in lake and river-water samples. The recovery by analysis of spiked samples was in the rage of 94∼108%.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 59 (10), 879-884, 2010

    The Japan Society for Analytical Chemistry

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