Electrochemical Study on Ion Transports Across a Bilayer Lipid Membrane in the Presence of Hydrophobic Ions and Ionophores

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  • SHIRAI Osamu
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

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Other Title
  • 疎水性イオン及びイオノホア添加時の脂質二分子膜を介したイオン透過の電気化学的研究
  • ソスイセイ イオン オヨビ イオノホア テンカジ ノ シシツ 2ブンシマク オ カイシタ イオン トウカ ノ デンキ カガクテキ ケンキュウ

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Abstract

The present paper describes a voltammetric study on ion transport from one aqueous phase (W1) to another (W2) across a bilayer lipid membrane (BLM) containing a hydrophobic ion, valinomycin (Val) or gramicidin A (GA). In particular, the ion-transport mechanisms are discussed in terms of the distribution of a pair of ions between aqueous and BLM phases. By the addition of a small amount of hydrophobic ion into W1 or/and W2 containing a hydrophilic salt serving as a supporting electrolyte, the hydrophobic ion was distributed into the BLM with a counter ion to hold the electroneutrality within the BLM phase. It was found that the counter ion transferred between W1 and W2 across the BLM upon applying a membrane potential. As for the facilitated transport of alkali ions across a BLM containing Val, which served as an ion carrier compound, it could be interpreted by considering not only the formation of an alkali metal ion-Val complex, but also the distribution of both the objective cation and the counter ion. In the case of the addition of GA as a channel-forming compound into the BLM, the facilitated transports of alkali ions across the BLM depended on the ionic species of the counter ions. It was discovered that the influence of the counter ion on the facilitated transports of alkali ions across the BLM could be explained by considering the hydrophobicity and the ionic radius of the counter ion.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 56 (7), 547-560, 2007

    The Japan Society for Analytical Chemistry

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