Application of Fluorescently-Labeled Glycosphingolipids to Metabolic Profiling in Single Cells Using Capillary Electrophoresis
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- Yoshimura Yayoi
- Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-1799 Copenhagen V, Denmark
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- Tanaka Hidenori
- Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-1799 Copenhagen V, Denmark
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- Dovichi Norman J.
- Department of Chemistry & Biology, University of Notre Dame, Notre Dame, Indiana, 46556, USA
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- Hindsgaul Ole
- Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-1799 Copenhagen V, Denmark
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- Palcic Monica M.
- Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-1799 Copenhagen V, Denmark
Bibliographic Information
- Other Title
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- 蛍光標識スフィンゴ糖脂質のキャピラリー電気泳動を用いた単細胞代謝解析への応用
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Abstract
Glycosphingolipids (GSLs) are important components expressed at the cell surface, where they play numerous roles in biological processes. The analysis of GSL metabolism is important but remains challenging in part due to the complexity of the components. To better understand the functions of GSLs on single cell scale we are developing an analytical method that uses fluorescently-tagged GSL analogs and capillary electrophoresis with laser induced fluorescence detection. We are applying this method to monitor glycolipid metabolism in single cells. In this article, we summarize our methodology, show examples of the chemo-enzymatic synthesis of glycolipid analogs, and provide some experimental data in single cell analysis.
Journal
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- Trends in Glycoscience and Glycotechnology
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Trends in Glycoscience and Glycotechnology 24 (138), 169-178, 2012
FCCA(Forum: Carbohydrates Coming of Age)
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Keywords
Details 詳細情報について
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- CRID
- 1390282679345180800
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- NII Article ID
- 10031146668
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- NII Book ID
- AA10995236
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- ISSN
- 18832113
- 09157352
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- NDL BIB ID
- 024179676
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed