Mutagenicity of a Reference Material Extracted from Urban Air Particles. A Collaborative Study with 5 Laboratories through 3 Years.

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  • 都市大気浮遊粒子から抽出された共通試料の変異原性　　５機関・３年間の共同研究から
  • トシ タイキ フユウ リュウシ カラ チュウシュツ サレタ キョウツウ シリョウ ノ ヘンイゲンセイ 5 キカン 3ネンカン ノ キョウドウ ケンキュウ カラ
  • -A Collaborative Study with 5 Laboratories through 3 Years-
  • ―5機関・3年間の共同研究から―

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Abstract

Mutagenicity of a reference material (RM) was analyzed with Salmonella (TA100, TA98 and YG1024) preincubation procedure under the collaboration of 5 laboratories through 3 years in Japan. The RM was prepared from urban air particle (total 9234.8 mg after the filtaration of 105 m3 air) . After ultrasonication with methylene chloride, the solvent was exchanged to dimethylsulfoxide. The RM was subdivided into 300 brown ampules (each 0.8 or 1.6 ml) at a central laboratory. Ampules were sent to each laboratories, and were storaged at -80°C through the project period (at least 3 years) . No significant variations of the mutagenicity were observed during the 3 years. Repeatability (intra-laboratory variance) of results ranged from 1.5 % to 55 % which seemed to depend on the bioassay conditions (tester strain and metabolic activation), whereas reproducibility (inter-laboratory variance) ranged from 27 % to 69 %. The reproducibility was shown to be similar to the repeatability from the results of two level nested analysis of variance in TA100 and TA98 assays.

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