Mutagenicity of a Reference Material Extracted from Urban Air Particles. A Collaborative Study with 5 Laboratories through 3 Years.
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- ENDO Osamu
- National Institute of Public Health
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- GOTO Sumio
- National Institute for Environmental Studies
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- MATSUMOTO Yutaka
- Hokkaido Institute of Environmental Sciences
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- ASANOMA Masaharu
- Nagoya City Public Health Research Institute
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- WATANABE Tetsushi
- Kyoto Pharmaceutical University
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- SERA Nobuyuki
- Fukuoka Institute of Health and Environmental Sciences
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- WAKABAYASHI Keiji
- National Cancer Center Research Institute
Bibliographic Information
- Other Title
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- 都市大気浮遊粒子から抽出された共通試料の変異原性 5機関・3年間の共同研究から
- トシ タイキ フユウ リュウシ カラ チュウシュツ サレタ キョウツウ シリョウ ノ ヘンイゲンセイ 5 キカン 3ネンカン ノ キョウドウ ケンキュウ カラ
- -A Collaborative Study with 5 Laboratories through 3 Years-
- ―5機関・3年間の共同研究から―
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Abstract
Mutagenicity of a reference material (RM) was analyzed with Salmonella (TA100, TA98 and YG1024) preincubation procedure under the collaboration of 5 laboratories through 3 years in Japan. The RM was prepared from urban air particle (total 9234.8 mg after the filtaration of 105 m3 air) . After ultrasonication with methylene chloride, the solvent was exchanged to dimethylsulfoxide. The RM was subdivided into 300 brown ampules (each 0.8 or 1.6 ml) at a central laboratory. Ampules were sent to each laboratories, and were storaged at -80°C through the project period (at least 3 years) . No significant variations of the mutagenicity were observed during the 3 years. Repeatability (intra-laboratory variance) of results ranged from 1.5 % to 55 % which seemed to depend on the bioassay conditions (tester strain and metabolic activation), whereas reproducibility (inter-laboratory variance) ranged from 27 % to 69 %. The reproducibility was shown to be similar to the repeatability from the results of two level nested analysis of variance in TA100 and TA98 assays.
Journal
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- Journal of Environmental Chemistry
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Journal of Environmental Chemistry 11 (3), 567-574, 2001
Japan Society for Environmental Chemistry
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Details 詳細情報について
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- CRID
- 1390282679345534208
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- NII Article ID
- 10009673999
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- NII Book ID
- AN10547099
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- COI
- 1:CAS:528:DC%2BD3MXnslejsbY%3D
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- ISSN
- 18825818
- 09172408
- http://id.crossref.org/issn/09172408
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- NDL BIB ID
- 5918322
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed