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- Wang Lai-Xi
- Institute of Human Virology and Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
書誌事項
- タイトル別名
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- The Amazing Transglycosylation Activity of Endo-.BETA.-N-Acetylglucosaminidases
- Endo-β-N-Acetylglucosaminidaseの驚異的糖転移能力[含 英語文]
- Endo ベータ N Acetylglucosaminidase ノ キョウイテキ トウ テンイ ノウリョク ガン エイゴブン
- The Amazing Transglycosylation Activity of Endo-β-N-Acetylglucosaminidases
- The amazing transglycosylation activity of endo-β-<italic>N</italic>-acetylglucosaminidases
この論文をさがす
抄録
Major advances have been made in exploring the trans-glycosylation activity of endo-β-N--acetylglucosaminidases (ENGases) for synthetic purpose. The exploration of synthetic sugar oxazolines as donor substrates for the ENGase-catalyzed transglycosylation has expanded the substrate availability and significantly enhanced the overall transglycosylation efficiency. On the other hand, site-directed mutagenesis in combination with activity screening has led to the discovery of the first generation ENGase-based glycosynthases that can use highly active sugar oxazolines as substrates for transglycosylation but lack hydrolytic activity on the ground-state products. ENGases have shown amazing flexibility in transglycosylation and possess much broader substrate specificity than previously thought. Now the ENGase-based chemoenzymatic method has been extended to the synthesis of a range of complex carbohydrates, including homogeneous glycopeptides, glycoproteins carrying well-defined glycans, novel oligosaccharide clusters, unusually glycosylated natural products, and even polysaccharides. This article highlights recent advances related to ENGase-catalyzed transglycosylation with a focus on their synthetic potential.
収録刊行物
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- Trends in Glycoscience and Glycotechnology
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Trends in Glycoscience and Glycotechnology 23 (129), 33-52, 2011
FCCA(Forum: Carbohydrates Coming of Age)
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詳細情報 詳細情報について
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- CRID
- 1390282679346876544
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- NII論文ID
- 10029582506
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- NII書誌ID
- AA10995236
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- COI
- 1:CAS:528:DC%2BC3MXht1Gksb%2FO
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- ISSN
- 18832113
- 09157352
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- NDL書誌ID
- 11218553
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可