Mutagenicity of the Chlorination Reactant of Cow Dung and Horse Dung

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Abstract

In the present study, the mutagenicity of chlorinated cow dung was measured by the Ames preincubation method using S. typhimurium TA100 and the TA98 strain. It was found that chlorination might induce mutagenicity in cow dung, and it could be detected without S9mix. Analysis revealed that the most reliable conditions for analyzing this effect, might be the use of extracts of dung chlorinated at 20°C for 2 hours with 6% chlorine solution. When the mutagenicity of five samples of chlorinated cow dung and three samples of chlorinated horse dung were measured under these conditions, there was no difference in mutagenicity between individual cows and individual horses. In general, mutagenicity sensitivity of the TA100 strain tended to be higher than that of the TA98 strain.

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