In Vitro Cytotoxicity Evaluation of Trichloroethylene and Tetrachloroethylene by the Pollen Culture Method.

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  • 花粉培養法を用いたトリクロロエチレン・テトラクロロエチレンの毒性評価法の検討
  • カフン バイヨウホウ オ モチイタ トリクロロエチレン テトラクロロエチレン ノ ドクセイ ヒョウカホウ ノ ケントウ

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We used a pollen culture method to evaluate the in vitro cytotoxicity of trichloroethylene (TCE) and tetrachloroethylene (PCE) in a liquid medium. Cytotoxicity was evaluated for various concentrations of TCE or PCE in the medium, in which pollen tubes of tea were grown. Inhibition of pollen tube growth was observed with the addition of more than 44.2 mg/l of TCE or 2.8 mg/l of PCE.<BR>The IC50 of TCE was about 71.6 to 140 mg/l, and for PCE about 5.9 to 12.7 mg/l This test can be performed easily and quickly and may be useful for in vitro cytotoxicity evaluation of these volatile compounds.

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